rna loading dye (New England Biolabs)

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New England Biolabs rna loading dye
Rna Loading Dye, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 498 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rna+loading+dye/RNA+Loading+Dye/pmc13092018-57-12-15
Average 97 stars, based on 498 article reviews

rna loading dye - by Bioz Stars, 2026-09

97/100 stars

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Article Title: Systematic screening of archaeal MazF homologs reveals Tth-MazF1, a versatile, sequence-specific ribonuclease from Thermococcus thioreducens
Article Snippet: Next, 10 μl of 2× RNA loading dye (NEB, B0363S) was added, and samples were heated at 70°C for 5 min. A total of 18 μl from each reaction was loaded onto a pre-cast 15% TBE-Urea polyacrylamide gel and electrophoresed at 180 V for 60 min. Gels were imaged using the Cy2 setting on the Amersham Typhoon scanner.

Article Title: Systematic screening of archaeal MazF homologs reveals Tth-MazF1, a versatile, sequence-specific ribonuclease from Thermococcus thioreducens.
Article Snippet: Next, 10 μl of 2 × RNA loading dye (NEB, B0363S) was added, and samples were heated at 70 ◦C for 5 min. A total of 18 μl from each reaction was loaded onto a pre-cast 15% TBE-Urea polyacrylamide gel and electrophoresed at 180 V for 60 min. Gels were imaged using the Cy2 setting on the Amersham Typhoon scanner.

Article Title: Systematic screening of archaeal MazF homologs reveals Tth-MazF1, a versatile, sequence-specific ribonuclease from Thermococcus thioreducens
Article Snippet: Following digestion, 10 μl of 2× RNA loading dye (NEB, B0363S) was added to each sample, which were then heated at 70°C for 5 min. A total of 18 μl from each reaction was loaded onto a pre-cast 15% TBE-Urea polyacrylamide gel and electrophoresed at 180 V for 60 min. Gels were imaged on the Amersham Typhoon scanner using the Cy2 setting.

Article Title: Systematic screening of archaeal MazF homologs reveals Tth-MazF1, a versatile, sequence-specific ribonuclease from Thermococcus thioreducens
Article Snippet: Following digestion, 10 μl of 2× RNA loading dye (NEB, B0363S) was added to each sample, which was then heated at 70°C for 5 min. A total of 18 μl from each reaction was loaded onto a pre-cast 15% TBE-Urea polyacrylamide gel and electrophoresed at 180 V for 60 min. Gels were imaged using the Cy2 setting on the Amersham Typhoon scanner.

Staining:

Article Title: PANDORA-seq reveals human sperm sncRNA signature endowed with sperm quality assessment
Article Snippet: .. Briefly, total RNA was denatured at 95 °C for 5 min with RNA loading dye (New England Biolabs, B0363S), separated by 15% denatured urea-PAGE, and stained with SYBR Gold (Thermo Fisher, S11494). .. 15–50 nucleotides (nt) were excised according to RNA ladders (New England Biolabs, N0364S; Takara, 3416), and RNA was extracted.

Article Title: Structure-informed mutagenesis identifies combinatorial contributions to mouse insulin receptor IRES function.
Article Snippet: The RNA was then cleaned using a Zymo RNA Clean & Concentrator-25 kit according to the manufacturer’s recommendations with the optional in-column DNase I treatment. .. The resulting RNA’s purity was assessed by NanoDrop and run on a 1% agarose 1× TBE gel using 2× RNA Loading Dye (New England Biolabs) and stained with 1 μg/mL ethidium bromide. ..

Incubation:

Article Title: Synthesis of highly complex phosphorothioate-modified oligonucleotides on microarrays.
Article Snippet: 19 pmol of each sample were prepared for gel analysis either in 1x DNase I buffer (NEB) as reference, or with additional 0.2 u/μL DNase I (NEB) to study degradation. .. All samples were incubated for 30 min at 37°C, mixed 1:1 with 2× RNA loading dye (NEB), heated to 70°C for 10 min and placed on ice before loading. .. The 12% PAGE gel was prepared using Rotiphorese Gel Stock solutions (CarlRoth) and supplemented with 7 M urea (Sigma).

Membrane:

Article Title: RNase P/MRP subunits chaperone telomerase holoenzyme assembly in fission yeast.
Article Snippet: .. The RNA was resuspended and mixed with an equal volume of 2× RNA loading dye (NEB, B0363S), heated at 75 °C for 5 min, and separated on a 4% (v/v) polyacrylamide (29:1)/8 M urea gel in 1× TBE buffer at 15W for 80 min. RNA was transferred to a Biodyne nylon membrane (Pall Corporation) at 400 mA for 1 h in 0.5× TBE. .. Crosslinking was performed using a Stratalinker (Stratagene) with 254-nm UV light at 120 mJ/cm2.

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